PERMFILE109666

• Permit No: C1981008A
• IBM Index Class Name: Permit File
• Doc Date: 12/11/2001
• Section_Exhibit Name: NH1 TAB 6 APPENDIX 6-3 CHEMICAL ANALYSIS PROCEDURES
• Media Type: D
• Archive: Yes

AVAILABLE PHOSPHOP.US IN MINE SOILS
(Modified method of Olsen, et a1, 1954)
Solutions:
1. Sodium bicarbonate (NaHCO ) solution, 0.519, adjusted to pH
8.5 with 1 M NaOH. Mineral oil added to avoid exposure to
the air; stored in a polyethylene container and made fresh
every 2 months.
2. Ammonium molt'§date, (NH ) Mo O 4 4H O, solution:
Dissolve l.5 g• {n 300 m~ ~f w~r~ disti~led and deionized
water. Filter if a.7.oudy and allow to cool. Gradually
add 342 ml of conc. HCL and mix. Dilute to 1 liter.
3. Concentrated SnCl 2H O solution: 10 g. of large crystals
dissolved in 25 m~. conc. HC1. Store in a brown glass
bottle in a refrigerator. Prepare fresh every Z months.
a) pilute SnCl add 0.5 ml of the conc. SnCl solution
to 66 ml. distilled and deionized water. 2Prepare
the dilute solution for each set of determinations.
• 4. Standard P solution: Weigh 0.4393 g. KH2PO4 into a 1 liter
volumetric flask. Add 500 mis. distilled and deionized
water and dissolve the salt. Dilute to 1 liter, and add
5 drops of toluene to reduce microbial growth.
5. Dilute P solution: pilute 20 ml. (pipet of the P solution
from 14) to 1 liter with distilled and deionized water.
This solution contains two micrograms of P per ml.
Procedure-
1. Add 1.OOg of 60 mesh rock or soil sample, and 20 ml of
Na HCO solution to a 50 ml Erlenmeyer flask. Stopper
the fl~sk.
2. Shake for 30 min., at 20°C, using a shaking speed of 2
on ~ Burrell Wrist Action shaker.
3. Centrifuge for ].5 minutes at 2,000 R.P.M.
4. Pipet 10 ml of supernate into a 25 ml volumetric flask and
dilute to volume. Transfer to a test tube.
5. Slowly add 5 ml of Ammonium Molybdate solution. Mix well.
6. Pipet 1 ml of dilute SnC12 solution into filtering flask,
and mix.
7. Read absorbance on a spectrophotometer a 660 mu, after
10-20 min.
6-97