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PARTI Page 9 Permit No. CO-00(10221 • A. TERDIS .AND CONDITIONS 4. Whine Effluent "toxicity Testing Reuuirements (Acute) - Outfnlls Otl?. 003. 0(15, 006 O(O Ol 1. Olt 0l3 Ol4 lllfi 017 Tesnne and Reportine Requirements Tests shall be done at the frequency listed m Part LB I Test results shall be reposed along with the Discharge Monitoring Report (DMR) submitted for the reporting period during which the sample was taken. (i.e.. WET testing results for the first calendar quarter ending March 31 shall be reported with the DMR due April 28.) The results shall be submitted on the Acute Toxicity Test report form, available from the Division. Copies of those reports are to be submitted to the Division along with the DMR. The perminee shall conduct acute WET tests as follows: for outfalls 002, 003, 006, 010 and 01 l - a 48-hour WET test using Ceriodaphnia sp.; (or out(all 005 - a 48-hour WET test using Daphnia magna; and for outfalls 012, 013, 014, Ol6 and 017 - a 48-hour WET test using Ceriodaphnia sp. and an acute 96-hour WET test using fathead minnows. Acute tests will be replacement static tests of a single effluent grab sample. The perminee shall conduct each acute WET test in general accordance with methods described in Methods for Measurine the Acute Toxicity of EfFluents and Receiving Waters to Freshwater and Marine Organisms, EPA/600/4-901027 or the most current edition, except as modified by the most current Division guidance document entitled Guidelines for Conducting Whole Effluent Toxicity Tests. b. Failure of Test and Division Notification An acute WET test is failed whenever the LCso, which represents an estimate of the effluent concentration which is lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less than or equal to 100% efFluent. The perminee must provide written notification of the failure of a WET test [o the Division, along with a • statement as to whether the Preliminary Toxicity Incident ("PTI')/Toxicity Identification Evaluation ("TIE") investigation or accelerated testing is being performed (see next section). Notification mtLSt be received by the Division within I4 calendar days of the demonstration of acute WET in the routine required [est. "Demonstration" for the purposes of Parts I.A.4.b., c., d. and f. means no later than the last day of the laboratory test. c. Automatic Compliance Schedule Upon Failure of Test 1(a routine acute WET test is (ailed, the following automatic compliance schedule shall apply. As part of this the perminee shall either: I. proceed to conduct the PTIlTIE investigation as described N Pan I.A.4 d., or ii. conduct accelerated testing using the single species found to be more sensitive. If accelerated testing is being performed, the permittee shall provide written notification of the results within 14 calendar days of completion of the "Pattern of Toxicity"/"No Toxicity" demonstration. Testing will be at least once every two weeks for up to five tests until 1) two consecutive tests fail or three of five tests fail, in which case a pattern of toxicity has been demonstrated or, 2) two consecutive tests pass or three of five tests pass, in which case no pattern of toxicity has been found. If no pattern of toxicity is found the toxicity episode is considered to be ended and routine testing is to resume. IF a pattern of toxicity is found, a PTI/TIE investigation is to be performed. !f a pattern of toxicity is not demonstrated but a significant level of erratic toxicity is found, the Division may require an increased frequency of routine monitoring or some other modified approach. d. PTIlTIE "Ihe results of the PTIlTIE investigation are to he received by the Division within 120 davs of the demonstration of acute WET in the routine test. as defined above, or it accelerated testing is performed, the • date the pattern of tonicity is demonstrated. A status report is to he provided to the Division at the 10 60 and 90 day points of the PTI/T~ investigation. The Division may extend the nine frame for invesugauon where reasonable justification exists. A request (or an euension must be made in writing and received prior to the I'_0 day deadline Such request must include a justification and supposing data (or such an extension. Corrected -~ - 72~/ 7 Amcndniem No I Issued 91 t t ry~ 1~G~ Eflecnve ;s iJ ~- ~., ..