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16 <br />upper Green River and lower Yampa River (Tyus 1987). ]~anigan and Tyus <br />(1989) estimated that from 758 to 1,138 razorback suckers inhabit the <br />upper Green River. Modde et al. (1996) report no significant decrease <br />in the population between 1982 and 1992, and the conti~lued presence of <br />fish smaller than 480 mm during the study period suggest some level of <br />recruitment. In the Colorado River, most razorback su~~kers occur in <br />the Grand Valley area near Grand Junction, Colorado; h~~wever, they are <br />increasingly rare. Osmundson and Kaeding (1991) repor•= that the <br />number of razorback sucker captures in the Grand Junction area has <br />declined dramatically since 1974. In 1991 and 1992, 23 adult <br />razorback suckers were collected from isolated ponds adjacent to the <br />Colorado River near DeBeque, Colorado (Burdick 1992). The existing <br />habitat has been modified to the extent that it impairs essential <br />behavior patterns, such as breeding, feeding, and sheltering. <br />Razorback suckers are in imminent danger of extirpation in the wild <br />The razorback sucker was listed as endangered October 23, 1991 <br />(56 FR 54957). As Bestgen (1990) pointed out: <br />"Reasons for decline of most native fishes in the Colorado River <br />Basin have been attributed to habitat loss due to construction of <br />mainstream dams and subsequent interruption or alteration of <br />natural flow and physio-chemical regimes, inundation of river <br />reaches by reservoirs, channelization, water quality degradation, <br />introduction of nonnative fish species and resulting competitive • <br />interactions or predation, and other man-induced disturbances <br />(Miller 1961, Joseph et al. 1977, Behnke and Benson 1983, Carlson <br />and Muth 1989, Tyus and Karp 1989). These factor:; are almost <br />certainly not mutually exclusive, therefore it is often difficult <br />to determine exact cause and effect relationships." <br />The virtual absence of any recruitment suggests a combination of <br />biological, physical, and/or chemical factors that may be affecting <br />the survival and recruitment of early life stages of razorback <br />suckers. Within the Upper Basin, recovery efforts endorsed by the <br />Recovery Program include the capture and removal of razorback suckers <br />from all known locations for genetic analyses and development of <br />discrete brood stocks if necessary. These measures have been <br />undertaken to develop refugia populations of the razorback sucker from <br />the same genetic parentage as their wild counterparts such that, if <br />these fish are genetically unique by subbasin or individual <br />population, then separate stocks will be available for future <br />augmentation. Such augmentation may be a necessary step to prevent <br />the extinction of razorback suckers in the Upper Basin. <br />McAda and Wydoski (1980) and Tyus (1987) reported springtime <br />aggregations of razorback suckers in off-channel habitats and <br />tributaries; such aggregations are believed to be asscciated with • <br />