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METHODS <br />In eazly June 1998, four reference sites were established within reclaimed azeas at Coal Basin Mine that <br />had become infested with noxious weeds. The reference sites were located within lazger infested azeas that <br />would be subject to weed control efforts over a several yeaz period. Size of the reference sites vazied, but <br />each was rectangular in shape, and mazked in the field by wooden or steel stakes at each comer. A <br />systematic sampling design was employed for data collection within each reference area. Repeated <br />sampling of the same azeas over the course of the control project was intended to provide an objective basis <br />for evaluating treatment success; recognizing that, because multiple replications of treatments and controls <br />were not employed, population trends could not be definitively linked to specific management variables. <br />Reference site locations, slope and aspect, and initial infestation severity, were chosen to be generally <br />representative of the larger treatment areas within which the reference azeas were located. Statistical <br />inference based on data from individual reference azeas cannot be extended to the larger treatment azeas. <br />However, the reference area data can be combined with visual evaluation of reference and larger treatment <br />areas to ensure a more objective assessment of treatment impacts, and to provide a sound basis for <br />professional judgement and management decisions. Sampling was conducted the 3d or 4t6 weak of June <br />each yeaz. <br />Reference Area #1 ("Sutey Pile Flat") is a SOm x 35m area on relatively flat topography located <br />immediately to the west of the Sutey Refuse Pile, within the "V" formed by a ranch road and a refuse pile <br />access road that diverge at that location. Each year of the study, ten, 35m cover transects were run, with <br />Sm spacing between transects. Two cover readings were made at 1.5 intervals along each transect, using a <br />tripod mounted oculaz point bar, giving a total of 4b cover points per transect. Live vegetation (current <br />year's growth for herbaceous vegetation) was recorded by species, along with litter, rock, and baze ground. <br />In 1998, density of combined thistle species wasestimated by counting live thistle plants within a Im by <br />35m quadrat along one side of each transect. In subsequent yeazs, houndstongue density was also <br />estimated. <br />Reference Area #2 ("West Slope Sutey") is a SOm x 25m area on the steep west facing slope of the Sutey <br />Pile, near the southwest cornet of the pile. Ten, 25m covet transects were run each year, with Sm spacing <br />between transects. Two cover readings were made at lm intervals alongthe transect, for a total of 50 cover <br />points per transect. Each year, density of combined thistle species andhoundstongue was estimated for the <br />south half of the reference area, by counting individual plants within 1xZ5mquadrats along one side of <br />each of the five transects within the south half of the reference azea. <br />Reference Area #3 ("Belt-line Flat") is located along the relatively flat surface of a segment ofbeltline <br />corridor, which was reseeded in the fall of 1997. The reference area is SOm x lOm, with ten, lOm transects <br />spaced at Sm intervals. Two cover readings were made at O.Sm intervals, for a total of 40 cover readings <br />per transect. Thistle density was estimated by counting thistle plants within 1x10mquadrats along one side <br />of each transect. <br />Reference Area #4 ("Belt-line Outslope") is not actually a reference area, but rather two pazallel50m <br />transects along the weed infested outslope of the beltline corridor, adjacent to Reference Area #3. One <br />cover reading per one meter interval was recorded, for a total of 50 points per transect. Cover readings for <br />each transect were made on the uphill side ofthe transect. Due to very high initial density of noxious <br />weeds, density counts were not attempted. Transects were staked at each end to ensure that the same <br />transect locations were read each yeaz. <br />