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SOP: Chain o(Custody, <br />Handling, Packing, Shipping <br />• tissue preparation procedure (biota; e.g., fillets, whole body), if applicable; and <br />• preservative added, if applicable. <br />3. Cover the label with clear packing tape to secure the label onto the container. <br />4. Check the caps on the sample containers to seal them tightly. <br />5. Wrap the sample container cap with cleaz packing tape to prevent it from becoming loose. <br />6. Place a signed custody seal label over the cap such that the cap cannot be removed without breaking the <br />custody seal. Alternatively, if shipping several containers in a cooler, custody seal evidence tape may be <br />placed on the shipping container as described below. <br />Packing Procedures <br />1. Using duct tape, secure the outside and inside of the drain plug at the bottom of the cooler being used for <br />sample transport. <br />2. Place each container or package in individual polyethylene bags (resealable-type) and seal. If a cooler <br />' temperature blank is supplied by the laboratory, it should be packaged following the same procedures as the <br />samples. If the laboratory did not include a temperature blank, do not add one since the sample temperature <br />will be determined by the laboratory using a calibrated infrazed thermometer. <br />3. Place 1 to 2 inches of cushioning material at the bottom of [he cooler. <br />4. Place the sealed sample containers upright in the cooler. <br />5. Package ice or blue ice in small resealable-type plastic bags and place loosely in the cooler. Do not pack ice <br />so tightly that it may prevent the addition of sufficient cushioning material. Samples placed on ice will be <br />cooled to and maintained at a temperature of approximately 4°C. <br />6. Fill the remaining space in the cooler with cushioning/absorbent material. The cooler must be securely <br />packed and cushioned in an upright position and be surrounded by a sorbent material capable of absorbing <br />spills from leaks or breakage of sample wntainers. ore: to comply with 49 CFR 173.4, filled cooler must <br />not exceed 64 pounds). <br />7. Place the completed chain-of-custody record(s) in a large resealable-type bag and tape the bag to the inside <br />of the cooler lid. <br />8. Close the lid of the cooler and fasten with packing tape. <br />9. Wrap strapping tape around both ends of the cooler. <br />10. Mark the cooler on the outside with the following information: shipping address, return address, "Fragile, <br />Handle with Care" labels on the top and on one side, and arrows indicating "This Side Up" on two adjacent <br />sides. <br />11. Place custody seal evidence tape over front right and back left of the cooler lid and cover with clear plastic <br />tape. <br />direros <br />SOP03-Sample Custody, Handling, Packing, antl Shipping-Mine.tloc <br />