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DISCUSSION <br />The D. magna test fails due to the creation of an LC50 concentration of 44.48%. <br />Possible reasons for this mortality could be the high alkalinity, high conductivity or the presence <br />of a large amount of total dissolved solids (TDS). Accelerated testing is the next step in fulfilling <br />permit requirements for the D. magna species. <br />There was no mortality seen in the fathead minnow test, so this species passes whole <br />effluent toxicity test requirements. <br />REFERENCES <br />EPA-821-R-02-012. 2002. Methods for Measuring the Acute Toxicity of Effluents and <br />Receiving Waters to Freshwater and Marine Organisms (fifth edition). 266 pp. <br />APHA/AWWANVEF. 1998. Standard Methods forthe Examination of Water and Wastewater. <br />20th Edition. American Public Health Association. <br />Hach Company. 2002. Hach Wafer Analysis Handbook. 4fh Edition. Hach Company, <br />Loveland, Colorado. 1260 pp. <br />The SeaCrest Group 4 <br />