My WebLink
|
Help
|
About
|
Sign Out
Home
Browse
Search
HYDRO31563
DRMS
>
Back File Migration
>
Hydrology
>
HYDRO31563
Metadata
Thumbnails
Annotations
Entry Properties
Last modified
8/24/2016 8:55:12 PM
Creation date
11/21/2007 2:00:28 AM
Metadata
Fields
Template:
DRMS Permit Index
Permit No
C1980007
IBM Index Class Name
Hydrology
Doc Date
8/2/2004
Doc Name
BioMonitoring Tests Report (April 28, 2004)
From
Mountain Coal Company, LLC
To
WQCD
Permit Index Doc Type
DMR’s
Media Type
D
Archive
No
There are no annotations on this page.
Document management portal powered by Laserfiche WebLink 9 © 1998-2015
Laserfiche.
All rights reserved.
/
18
PDF
Print
Pages to print
Enter page numbers and/or page ranges separated by commas. For example, 1,3,5-12.
After downloading, print the document using a PDF reader (e.g. Adobe Reader).
View images
View plain text
INTRODUCTION <br />Biomonitoring provides an effective method for testing the toxicity of effluents from <br />municipal and industrial discharges. One of the advantages of Biomonitoring is the ability to <br />test complex effluents containing a broad range of contaminants. These methods generate data <br />that cannot be obtained solely from chemical analyses. <br />Biomonitoring tests were conducted for Mountain Coal Company in April 2004. In <br />accordance with accepted Environmental Protection Agency (EPA) and State of Colorado <br />procedures, acute tests were performed using Daphnia magna and the fathead minnow <br />(Pimephales promelas). This report details the results of the tests. <br />MATERIALS AND METHODS <br />Sample Collection <br />A sample of the 017 discharge was collected into one-gallon plastic containers at 09:00 <br />on April 7, 2004. The effluent was packed in an ice chest and shipped overnight to the lab <br />where it arrived at 09:26 on April 8, 2004. At SeaCrest lab the samples were refrigerated at <br />4° C when not in use. The Chain of Custody forms, documenting sample collection and lab <br />arrival times, are included in Appendix 1. <br />Test Organisms <br />Daphnia magna were chosen as the invertebrate species most able to tolerate the high <br />dissolved solids levels found in Mountain Coal samples. The Daphnia magna used in the test <br />were cultured in the SeaCrest laboratory. The daphnids were less than 24 hours old at the start <br />of the test. The daphnids were fed prior to the test start-up but not during the 48-hour test. <br />Daphnids are fed a mixture of yeast, cereal leaves, and trout chow (YCT); and the green <br />microalga, Selenastrum capricornutum. <br />The fathead minnow larvae are obtained from adults held in ten and twenty gallon <br />aquaria. The females deposit their eggs on the undersurface of split PVC pipe sections from <br />which they are collected daily. The eggs are transferred from the tiles to aerated containers <br />where they hatch after 3-4 days. The larvae are fed newly hatched brine shrimp (Artemis sp.) <br />at least twice daily. Nine-day-old fish were used in the present test. <br />All in-house organisms are tested at least monthly in a reference toxicant test using <br />sodium chloride to confirm. their overall health. <br />The SeaCrest Group 2 <br />
The URL can be used to link to this page
Your browser does not support the video tag.