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W.E.T. rec. <br />Wafer & Environmental Testing /nc. 479 Wesl SO North, American Fork Ulah 84003 /8011756-8397 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: October 2, 1997. <br />PERMITTEE NAME: Western Fuel Colorado SIGHT ID: New Horizon Mine <br />TEST /Anima!/AgeJ: Ceriodaphnia <24 hours. <br />OUTFACE NO: 007 <br />SAMPLE (Date/Type): 9/26//97 Grab. <br />DATE/TIME TEST BEGAN: 9/27/97 5:40 p.m. DATE/TIME TEST COMPLETED: 9/29/97 5:30 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted E®uent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Auimal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />PH <br />Dilution Water <br />Receiving Water <br />Q4 hours. <br />5 organisms/l5 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hottrs. <br />Reconstituted water LI 5% hardness of the receiving <br />water). <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO signi5cant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincec ly, f <br />~~' ati~d- <br />Lee Rawlings / <br />Water & Environmental Testing, Inc. <br />Enclosure <br />