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PART I <br />Page No. 5 <br />Permit No. CO-0000132 <br />A. DEFINTI'ION OF EFFLUENT LIMI'PATIONS <br />2. Acute WET Testine - Outfa11011 <br />(a) Testine and Reoortina Requirements <br />Tests shall be done at the frequency listed in Part I.B.1. Test results shall be reported along with the Dischazge <br />Monitoring Report (DMR) submitted for the reporting period during which the sample was taken. (i.e., WET testing <br />results for the fast calendar quarter ending Mazch 31 shall be reported with the DMR due April.28.) The results shall <br />be submitted on the Acute Toxicity Test report form, available from the Division. Copies of these reports are to be <br />submitted to both the Division and EPA along with the DMR. <br />The permittee shall conduct an acute 96-hour WET test using fathead minnows. Acute tests will be replacement static <br />tests of a single effluent grab sample. The permittee shall conduct each acute WET test in general accordance with <br />methods described in Methods for Measurine tite Acute Tozicirv of Effluents and Receivine Waters to Freshwater and <br />Marine Organisms, EPA/600/4-90/027 or the most current edition, except as modified by the most current Division <br />guidance document entitled Guidelines for Conductive Whole Effluent Toxicirv Tests. <br />(b) Failure of Test and Division Notification <br />An acute WET test is failed whenever the LCso, which represents an estimate of the effluent concenvation which is <br />lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less than or equal to 100% <br />effluent. The permittee must provide written notification of the failure of a WET test to the Division, along with a <br />statement as to whether the Preliminary Toxicity Incident ("PTI")/Toxicity Identification Evaluation ("TIE") <br />investigation or accelerated testing is being performed (see next section). Notification must be received by the <br />Division within 14 calendar days of the demonstration of acute WET in the routine requved test. <br />"Demonsva[ion" for the purposes of Pans I.A.2.(b),(c),(d) and (f) means no later than the last day of the laboratory <br />test. <br />(c) Automatic Compliance Schedule Upon Failure of Test <br />If a routine acute WET test is failed, the following automatic compliance schedule shall apply. As pan of this the <br />permittee shall either: <br />(i) proceed to conduct the PTllTIE investigation as described in Pan I.A.2.(d), or <br />(ri) conduct accelerated testing using the single species found to be more sensitive. <br />If accelerated testing is being performed, the permittee shag provide written notification of the results within 14 <br />calendar days of completion of the "Pattern of Toxicity"/"No Toxicity" demonstration. Testing will be at least <br />once every two weeks for up to five tests until 1) two consecutive tests fail or three of five tests fail, in which case a <br />pattern of toxicity has been demonsvazed or, 2) two consecutive tests pass or three of five tests pass, in which case no <br />pattern of toxicity has been found. If no pattern of toxicity is found [he toxicity episode is considered to be ended and <br />routine testing is to resume. If a pattern of toxicity is found, a PTI/TIE investigation is to be performed. If a pattern <br />of toxicity is not demonsvated but a significant level of erratic toxicity is found, the Division may require an increased <br />frequency of routine monitoring or some other modified approach. <br />See Part I.C. for Defmitions. <br />Amendment No. 5 Issued Effective <br />ixoev t+ama+ i,, iw <br />