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~~` W.E.T. Inc. <br />Water & Environmental Testin¢ /nc. 479 Wes[ SO North. American Fork U[ah 84003 /807)756-8397 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: December 23,1997 <br />PERMITTEE NAME: Western Fuel Colorado SIGHT H): New Horizon Mine <br />TEST (Anima!/Age): Ceriodaphnia <24 hours. <br />OUTFACE NO: 007 <br />SAMPLE (Date/Type): 12/9/97 Grab. <br />DATE/TIME TEST BEGAN: 12/10/97 3:00 p.m. DATE/1'IME TEST COMPLETED: 12/12/97 4:00 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted E6luent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Auimal Age at Test Start <br />Number of Orgaaisms/Dilutioo Volume/Replicates <br />Food <br />Aeration <br />Dissolved Orygeu <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Q4 hours. <br />5 organisms/15 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + i degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±15% hardness of the receiving <br />water). <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere) <br />C~~ <br />Lee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />