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W.~'. T'. Inc. <br />III IIIIIIIIIIIII III <br />999 <br />,.; <br />Water & Environmental Testine Jnc. 479 Wes! SO North, American Fork Utal+ 84003 (801)758397 <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: December 23, 1997 <br />PESiiV1lTTEE NAME: Western Fuels Colorado SIGHT ID: New Horizon Mine <br />TEST (AnimaUAge): Fathead Minnow, 7 days. <br />OUTFACE NO: 007 <br />SAMPLE (Date/Type) 12/9/97 Grab. <br />DATE/TIME TEST BEGAN: 12/10/97 3:45 p.m. DATEfI'IME TEST COMPLETED: 12/14/97 4:15 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Aaimal Age at Test Start <br />Number of Orgaoisms/Dilutiou Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Fathead Minnow, 7 days. <br />l0 organisms/150 mL/2 replicates <br />Fed before starting test end at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±PS% hardness of the receiving <br />water). <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheen and statistical reports. <br />~mceret <br />~v <br />ee Rawlings <br />Water & Environmental Testing, inc. <br />Enclosure <br />