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Client: Mountain Coal Company CO - 0038776 SCG Project No.: 306048 <br />Site: West Elk Mine Outfall 017 <br />RESULTS <br />The data sheets for the acute tests aze located in Appendix 2. Table 1 summarizes the <br />results of Daphnia magna tests with the treated and untreated effluent. All treated effluents <br />reported LCSOs of >100% as did the untreated sample. <br />Survival in the Fathead minnow test was 88% in the 100% effluent concentration. <br />Survival ranged from 93% - 100% in the remaining effluent concentrations. Control survival was <br />95%. <br />DISCUSSION <br />The sample collection showed no effects of toxicity to Daphnia magna in both the treated <br />and untreated effluent samples. -The dischazge passes WET (Whole Effluent Toxicity) test <br />guidelines for this species for this sampling period. <br />No significant toxicity was measured in the Fathead minnow test. The sample passes <br />WET requirements for this species for this sampling period. <br />A series of tests were performed in conjunction with the original effluent as part of the <br />permit requirements to confirm the effects of TDS against other possible contaminants. In this <br />case, the received sample did not show toxicity in the quarterly test. This result was consistent <br />with fmdings from the alkalinity adjustment test series. <br />The SeaCrest Group 7 <br />Table 1. Daphnia magna survival and LC50 calculations for treated/untreated effluent. <br />