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Client: Mountain Coal Company CO - 0038776 <br />Site: West Elk Mine Outfa11017 <br />SCG Project No.: 306048 <br />Acute Tonicity Test Summary <br />Test: • 48-hour static renewal using Daphnia magna <br />• 96-hour static renewal using Fathead minnows (Pimephales <br />promelas) <br />Client: <br />Mountain Coal <br />Test Procedure Followed: EPA-821-R-02-012 (2002) <br />Sample Description: 306048 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection <br />Receipt <br />Receipt <br />0900 01-19-2006 0930 01-20-2006 <br />3' s ;7s ~. gym. -7 4 ~ <br />;~:'~ .., m{~~ ~~ ~' r ,~ t ~ l~~ fin' <br />~. <br />Test Initiation Time ~ gas 5 ~ ~ <br />~q ~.1~Pl?± rhagn <br /> <br />1630 ~ ~~ S ~+ ~ <br />x a Fat~_~il~~ <br />~... <br />1205 <br />Test Initiation Date 01-20-2006 01-20-2006 <br />Test Completion Time 1550 1225 <br />Test Completion Date 01-22-2006 01-24-2006 <br />Dilution Water: • Moderately hard laboratory reconstituted water <br />Test Organism Source: • Daphnia magna <br />The SeaCrest Group <br />• Fathead minnows <br />The SeaCrest Group <br />Reference Toxicant: • Sodium Chloride <br />The SeaCrest Group 3 <br />