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THE SEACREST GROUP <br />Quality Assurance Check List -Acute and Chronic Whole Effluent Toxicity Test <br />Client: Mountain Coal Company <br />Stark Date of Test: April 29, 2004 <br />SeaCrest Sample Number: 304108 <br />Species Tested: Daphnia magna <br />Sample received in lab properly preserved (4° C)? N* <br />Sample delivered on ice or equivalent? Y* <br />Test initiated within 36 hours of collection? Y <br />Test protocol conforms to CDPHE guidelines: D. magna? Y <br />Test protocol conforms to CDPHE guidelines: P. promelas? NA <br />Average Test Temp + 1 o C: D. magna? Y <br />Average Test Temp ± 1 o C: P. promelas? NA <br />DO level >4.0 mg/L; no supersaturation: D. magna? Y <br />DO level >4.0 mg/L; no supersaturation: P. promelas? NA <br />Mortality in control <10%, <20% for chronic: D. magna? Y <br />Mortality in control <10%, <20% for chronic: P. promelas? NA <br />Daphnia magna neonates in control <24 hours old? Y <br />P. promelas larvae in control 1-14 days old in acute, < 1 day old in chronic? NA <br />Appropriate reference toxicity test conducted? Y <br />Lab Ref. Tox. test results within the confidence limits for the lab? Y <br />"` sample was at 16.0° C upon delivery. <br />D Sample was packed in Blue Ice. We have found that blue ice does not seem to adequately <br />chill the effluent samples over long periods of time. Ice has been found to give better results <br />in holding temperatures at or below the required 4° C. <br />Signature Date S~ l l3 / o'{ <br />Position: Senior Scientist <br />