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ivies i n~i~a <br />A two species acute bioassay was conducted with effluent from the West Fork of Sylvester <br />C.tllch, ntltfall 017. An effluent grah Samnle wag collected nn January 10, 2002, stored nn ice, <br />and delivered to Cortmmercial Testing and Engineering Co. lCTRrEI nn the following day. The <br />ef}lilent Sample was received at a temperati!re of 10.0 C and agcig_ C'T~E Samnle tracking <br />° ned <br />;lumber v~2-i ill vvi. Tile biva$$ay wag iiutlated appraximately 3ti,i hvurg aPer SarTipie <br />collection. <br />Testing was c~ndtlcted in accordance with the most recent nmtncols sneci_fi_ed by the Water <br />Qtaality Control Division of the Colorado Department nfHealth (199R1, outlined in Tahle 1. <br />RagtC CharaeterlStieg of the eiiiuent acid dlita?n~:g were measured ag ivlly vv g: <br />Charactet;gtic Method~F:ntrinment RpAMethnd <br />e ivalirity <br />Amlll(7111a <br />l;hlnrlne, Total Residual <br />C nndtlctivity <br />Dissolved Oxygen <br />Hardness <br />nH <br />Tempe. afore <br />Titrimetrc \epu~?,1 louo~ <br />L`QCllat All`61 JIJ Method <br />y <br />LaMotte STC-Ci Colorimeter <br />Orion Model 150 Cond. Meter <br />Orion Model R50 DO Meter <br />Titrimetric (APHA, 19$91 <br />Fisher Model 925 pN Meter <br />?`IISTZ calibrated thermometer <br />EPA 310.1 & 130.2 <br />EFA 35C. i <br />EPA accepted <br />EPA 120.1 <br />EPA 350.1 <br />F_.PA 31p.i ~ 130.2 <br />EPA 150.1 <br />EPA accepted <br />The tests exnnsed danhnicis, Dgnhnia magna, to five concentrations of e_fFltient, 100%, 75%, <br />$0% 2S% and 1.9%°, prepared using reconstituted lahnraYory water a1 22S mg/L CaCOs. <br />Additionally; the tests exposed fathead minnows, Pimenhales nromelas; to five concentrations of <br />effluent, 100%, 75%, 50%, 25%, and 1.9%, prepared using reconstituted laboratory water at 110 <br />mg/L. CaCO,. Control replicates were exposed to 100% laboratory water. <br />The Pmhit method determined the L.C~~'s, where applicable. Si_gni_ficant differences in mortality <br />were analyzed using Steel's Many-One Rank tests. <br />' American AiLc 1-icalth Association <br />z Natnnal InsLtu?e Ot ~3rlrlarric 2nri Trrllltningv <br />COMMERCIAL TESTING & ENGINEERING CO. <br />=-~ Ltd <br />)riginal Watermarked For Vour Protection <br />