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NARRATIVE <br />• CLIENT <br />Beaz Coal Company, P.O. Box 1754, Paonia, CO 81428 <br />• DATE OF REPORT <br />January 27, 2000 <br />• PERMTT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water: <br />CO-0044377 <br />Effluent Grab <br />01/18/00 0945 <br />01!19/00 0930 <br />Reconstituted Laboratory Water <br />• TEST ORGANISMS <br />Ceriodaphnia dubia <br />Daphnia magna <br />Age: <24 hrs <br />Age: <24 hrs <br />• PROCEDURE <br />Acute, 48 hour bioassays as outlined in the most recent protocols specified by the <br />Water Quality Control Division of the Colorado Department of Health (1998), with <br />reference to the EPA's Methods for Aquatic Toxicity Identification Evatuations. Phase I <br />Toxicity Characterization Procedures. (EPAl600/3-88/034 September 1988). <br />• DISCUSSION <br />CT&E conducted a Preliminary Toxicity Identification evaluation to confirm Total <br />Dissolved Solids toxicity. Although the results aze not conclusive, the derived evidence <br />strongly indicates that Total Dissolved Solids is contributing to acute toxicity towards <br />Ceriodaphnia dubia. <br />