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iii iiiiiiiiiiiii iii <br />W. ~' . T. Inc. 999 <br />Water d Envtronntmtal Tatln>t lne. 399 R'at J200 North. B Oren. Utoh 8~l057 /801)235-971 Faz1801)335-9560 <br />~- <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: Mazch 25, 2000 <br />PERMITTEE NAME: Western-Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 001 <br />TEST (AnimaUAgeJ: Fathead Minnow, 7 days. SAMPLE (Date/fypeJ: 3!20/00 Grab. <br />DATE/1'IME TEST BEGAN: 3!21/00 11:15 a.m. DATE/1'1ME TEST COMPLETED: 3!25/00 12:30 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted efFluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />SUMMARY <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />Fathead Minnow, 7 days. <br />10 organisms/I50 mL/2 replicates <br />Fed before starting test ahd at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water `I S% hardness of the receiving <br />water). <br />None was supplied. <br />Control, 6.25, 12.5, <br />pH <br />Dilution Water <br />Receiving Water <br />Sam le Concentt <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere , <br />~~W ~~ ~~ <br />Lce Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />