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W.~'. T. Inc. <br />MAR 3~0 2007 <br />III IIIIIIIIIIIII III <br />999 <br />Acute Whole Effluent Toxicity Report <br />Fathead Mrnnow <br />DATE: March 25, 2000 <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />PH <br />OUTFACE NO: 007 <br />SAMPLE (Dafelfype): 3/10/00 Grab. <br />DATE/I'IME TEST COMPLETED: 3/25/00 12:00 p.m. <br />Fathead Minnow, 7 days. <br />10 organisms/I50 mU2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ! I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±I S% hardness of the receiving <br />water). <br />None was supplied. <br />Dilution Water <br />Receiving Water <br />Sam le Concentt <br />SUMMARY <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine <br />TEST (AnimaUAge): Fathead Minnow, 7 days. <br />DATE/I'IME TEST BEGAN: 3!21/00 11:30 a.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Results: X .Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />~e Rawlings <br />Wa[er & Environmental Testing, Inc. <br />Enclosure <br />