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I - V.E. T. Inc. <br />Water & Environmental Testing Inc 23S West 400 South. American Fork. Utah 84003 (8011763-0660 Fax/80I1763-0440 <br />- Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE; September 26, 2001 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 001 <br />TEST (AnimaUAge): Ceriodaphnia <24 hours. <br />DATElTIME TEST BEGAN: 9/19/01 2;30 p.m. <br />SAMPLE (Date/Type): 9/18/01 Grab. <br />DATElTIME TEST COMPLETED: 9/21/01 2:10 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period <br />Survival was measured to determine if Acute Toxicity was present in the sample. <br />TestStart <br />Number of Organisms/Dilutioa Volume/Reptic <br />Aeration _ _ <br />Dissolved.Oxygeu _ _ __,^ <br />Water Replacement <br />Tempei•sture ~ _ __ _ ~ _ <br />Photo Period <br />~pH ,~ ~._' ~ , , <br />Dilution Water <br />., <br />rtes 5 ort;anisms/15 mL/4 replies <br />None <br />Renewed <br />16 hours li t 8 hours dazk. <br />---,-- <br />:, _Measured~ini~kan`dfat~24 <br />Reconstituted lab water aoo <br />200 me/L hardness. <br />SUMMARY P <br />Results: LX ,Pass ~ Fail <br />There was NO significant effect on survival. <br />Enclosed aze data sheets and statistical reports. <br />Sincerely, <br />e Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />~ ' <br />Page 4 of 8 <br />