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W E. T. Inc. <br />Water & Environmental Testine Inc. 235 West 400 South. American Fork. Utah 84003 (801)763-0660 Faz(8011763-0440 <br />~l <br />Acute -Whole Effluent Toxicity Report - <br />Fathead Minnow <br />DATE: September 26, 2001 <br />-~~ <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine <br />TEST (Animal/Age): Fathead Minnow, 5 days. <br />OUTFACE NO: 001 <br />SAMPLE (Date/Type): 9/18/01 Grab. <br />DATE/TIME TEST BEGAN: 9/19/01 3:00 p.m. DATE/TIME TEST COMPLETED: 9/23/01 6:30 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />~Ammal~`Age'atTesfStart ' <br />"+'' ~`~` ~ <br />.. <br />''Fathead~Iv'linnow {5`3a s. <br />Y <br />,_ _ <br />Number of Organisms/Dilution Volume/Replicates IO organisms/150 mI./2 replicates <br />'~ ~ <br />' <br />~ Fed before ~farhng testand at 48 o e c <br />~ <br />~ - ~?_k <br />Food .K <br /> <br />Aeration None required. <br />_ <br />:~Dtssol <br />ed <br />gen <br />~~r~ <br />0 ~Meas'ureddailv:old/new;t <br />": "` <br />v <br />~~~~_ ._ _- -., <br />_ .: <br />~ <br />_ry • <br />Water Replacement Renewed daily. _ <br />:Temperature _, _;,_t ~ ~~; ~~20,~+ 1'de'ge'es C -'' „, <br />Pboto Period 16 hours light 8 hours dazk. <br />_ <br />!P$ .~>~ - -~_.. ~z Y ' a Iv1~~"easured"'nnthallv an ~~ o <br /> Reconstituted lab water approx. 200 mg/L hazdness. <br />Dilution Water <br />K:." <br />celi~ia>;WVater ~ ~,~ '"'+' ` <br />ne <br />Sam le Concentrations Control 6.25 12.5 25 50 100%. <br />SUMMARY <br />^~- IY+ <br />Results: ~y~Xts'1 Pass ~t~~~ Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />~~ ~~J (~ <br />Rawlings <br />Water & Environmental Testing, Inc. <br />O <br />Enclosure <br />Page 2 of 8 <br />