Laserfiche WebLink
<br />INTRODUCTION <br />Western Fuels has previously conducted biomonitoring tests using Daphnia <br />magna and fathead minnows (Pimephales promelas). During August, 1992, a <br />sample of the 8th East roof water was tested using the above species as well <br />as Ceriodaphnia dubia to test the potential toxicity of this water. The present <br />report describes the results of these tests. <br />MATERIALS AND METHODS <br />Sample Collection and Handling <br />Grab samples of the roof water was collected in 1-gallon plastic containers <br />on August 13, 1992. The samples were delivered to T.H.E.'s laboratory via an <br />overnight express service where they were refrigerated at 4° C until testing. A <br />chain of custody form showing collection and lab arrival times is included in <br />Appendix 1. <br />Dilution Water <br />Reconstituted laboratory water was used as the source of dilution water and <br />a control. This water was prepared by adding sodium bicarbonate, magnesium <br />sulfate, calcium sulfate, and potassium chloride to deionized water. The water <br />was mixed to make a hard water. <br />Source and Acclimation of Test Organisms <br />Daphnia magna and Ceriodaphnia used in the tests came from in-house <br />cultures. These animals were less than 24 hours old at the start of the test. <br />Fathead minnows, Pimephales promelas, were also used in the test. These were <br />4-5 day old organisms which had been obtained from in house cultures. All in- <br />house organisms used in the lab are tested against a reference toxicant at least <br />monthly to determine overall health. <br />Quality Control/Quality Assurance <br />Various QA/~C procedures were utilized by T.H.E. in conducting the <br />biomonitoring tests. These included the use of chain of custody forms to <br />document sample receipt and times as well as to insure sample integrity. <br />Analytical tests followed standard methods and included the use of standards to <br />calibrate equipment and assure proper operation. Tests were conducted in an <br />incubator maintained at a 20°C. Light regimes for tests followed a 16 hour light, <br />6 hour dark cycle. <br />Sample Preparation <br />Five concentrations (100, 75, 50, 25, and 12°b) and a control consisting of <br />100°'° dilution water were used to test the effluent. Exposure concentrations <br />were prepared by mixing effluent with the reconstituted water. <br />