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W.~~.1: III IIIIIIIIIIIIIIII <br />999 <br />,._, <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />JUN 2 11999 <br />999---- <br />PERMITfEE NAME: WesteraFuels Co. (New Horizon Mine) NPDES NO: 007 OUTFACE NO: <br />TEST (Anima!/Age): Fathead Minnow, 7 days. SAMPLE (Datelfype): 6/8/99 Grab. <br />DATE!I')ME TEST BEGAN: 6/9/99 1:50 p.m DATEITIIVIE TEST COMPLETED: 6/13/99 2:00 p.m <br />TEST CONDTI'IONS <br />Fathead Minnow larvae wtrre exposed to diluted effluent az specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Tonicity was present in the sample. <br />Mimal Age at Test Start <br />Number of Orgaoisms/Dilution Volume/Replicates <br />SUMMARY <br />Food <br />Aeration <br />Dissolved O:ygen <br />Water Replace_me_ot <br />Temperature <br />Photo Period <br />PH <br />Dilution Water <br />Receiving Water <br />Sam le Cooceotr <br />Fathead Minnaw, 7 days. I <br />10 organisms/150 nil,/2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + 1 degrees C. ~ _ <br />16 hours light 8 hours dark <br />i <br />Measured initially and at 24 hours. <br />Reconstituted water (±15% hardness of Ure receiving <br />water). <br />None was supplied <br />Control, 6.25, 12.5, 25, 50, 100 <br />Rewlts: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely <br />' 1"."" <br />Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />