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PART I Page 15 Permit No. COG~00000 B. TERMS AND CONDITIONS 2. Effluent Limitations (coat.) I. WET Testing: The Division will r~amt~e each dischazge application on a case by case basis. In cases where the data and circumstances justify, the Division may determine that WET testing be required under the general permit for certain facilities. Similazly, the Division may determine that the WET testing frequrnry be either increased or decreased from the normal quarterly monitoring depending on the analytical data, durazion of dichazge, flow rates, or other factors that the Division deem relevant. As a condition of the permit, the permittee maybe required to conduct routine monitoring for acuu toxicity using two species, Ceriodaohnia sp. (water flea) and fathead minnows. Acute toxicity occurs whrn a species mortality in any dilution of effluent (including 100% efflurnt) exceeds 50 % for either species, or there is a statistically significant difference in the mortality observed for either species between the control and any efflurnt concentration. The monitoring frequency for acute WET tests shall be gtarterly, wmmeacing with the first full calendar quarter following the permit effective date. Quarterly test results shall be reported on a Quarterly DMR along with the Discharge Monitoring Reports (DMRs) submitted for the end of the reporting calrndaz quarter (i.e., WET testing results for the ralrndaz quarter ending March 31 shall be reported with the DMR due April 28, with the remaining WET testing reports submitted with DMRs due each July 28, October 28 and January 28). In addition to the WET test reporting DMR, the permittee shall submit CDPS WET Test Report Forms (generally completed by the laboratory for each species). Copies of these reports are to be submitted to both the Division and the EPA. The permittee shall conduct each acute WET test in grneral accordance with methods described in Methods for Measutine the Acute Tonicity of Efflurnts and Receivine Waters to Freshwater and Marine Or¢anisms, EPA1600/4-901027 or the most current edition, except as modified by the most current Division guidance document entitled Colorado Water Quality Control Division Biomonitorine Guidance Document (July 1993). The permittee shall conduct an acute 48-hour WET test using Ceriodanhnia sp., and an acute 96-hour WET test using fathead minnows. Acute tests will be replacemrnt static tests of a single effluent grab satuple. Should acute tonicity be detected, the permittce must provide written notification of the failure of a WET test to the Division, along with a statement as to whether the Preliminary Toxicity Incidrnt ("PTI')/Toziciry Identification Evaluation ("TIE") investigation or accelerated testing is being performed. Notification must be recrJved by the Division within 14 calendar days of the d®oaslration of acute WEl' is the routine required test: 'Demonstration' means no later than the last day of the laboruory test. If a routine acute WET test is failed, the permittce shall either: (1) proceed to conduct the PTIlI7E investigation as described below or (2) conduct accelerated testing using the single species found m be more sensitive.