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~W. ~'. T. Inc. <br />I <br />III Illlllllllllllll IIU, <br />20~ <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: December 14, 1999 <br />PERM[TTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (AnimaUAge): Fathead Minnow, 7 days. SAMPLE (Dare/7ypeJ: 12/7/99 Grab. <br />DATE/TIME TEST BEGAN: 1218199 1:00 p.m. DATEJI'IME TEST COMPLETED: 12/12/99 1:50 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted ettluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />'Agimal Ageet Test Start ' <br />Number of Organisms/Dilution Volume/Replicates <br />Food: <br />Aeretion <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Pboto Period <br />PH <br />Fadtead Minnow, '7 days, ..:.:. <br />10 organisms/150 mLR replicates <br />Fed: 6et'ore starting :test a f d at 48 .hours,.newly hatched . <br />brine shrimp:. ~ <br />None required. <br />.Measured daily old/new. ' <br />Renewed daily. <br />20.± 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±5% hardness of the receiving <br />water). <br />None was supplied. <br />Dilution Water <br />Reeeivimg Water <br />Sam le Concend <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data shcets and statistical reports. <br />Sincere , <br />G~ <br />Lee Rawlings <br />Water & Environmental Testing, lnc. <br />Enclosure <br />