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SGS <br />' ADUNE55 At L l.l:)n HE SPONOF NC E 10' <br /> WMMEnfIAL ifi5 f4JG M1 R:OINFER~N9 CO <br /> 4fiid~PAN1$F rNEFr SW rF. L1~2W <br /> :?ENVEn CO 80.'99 <br /> reL twat are-Anz <br /> <br />Table of Contents <br /> <br />Abstrac-t Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' Pg. 10 <br />Abstract <br />SGS Minerals Services Division conducted a two species acute bioassay on Westem Fuels New Horizon <br />Mine effluent discharge. This discharge has been designated as outfall 007. The purpose of this <br />bioassay was to determine if the facility complies with the acute WET portion of the Colorado State <br />Industrial General Minimal Discharge Permit. The lest organisms utilized were the fathead minnow <br />(Pimephales promeias) and cladoceran (Daphnia magna). Test results indicate that the effluent was not <br />acutely toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is in compliance <br />with the acute WET portion of its Colorado State Discharge Permit for the first quarter of 2004. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />outfall 007. An effluent grab sample was collected on March 2, 2004, stored on Ice, and delivered to <br />SGS Minerals Services Division on the following day. The effluent sample was received al a temperature <br />of -2.0 C and assigned sample tracking number 072-8495-001. The bioassay was initialed <br />approximately 27 hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1, Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the ctadocerans, Daphnia magna, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%. 75°i°, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water at 120-205 mgiL CaCO~ Control replicates were exposed to 100% laboratory water <br />Statis;;czllly. the Binomial method determined the LCso's'. Significant differences in mortality were <br />analvzcrl using the Steel's h1~nv-One Rank test. <br />Supplemental loans Include statistical analysis anA chain-o4custody loans <br />LC% - an estimate of the concentralinn Iothai to 50 ~, of the organisms m the time prescdbod by tho lost <br />m,~. ~•„il~a~; r•r-,I ,ia,~:tnll lr: ninnd 'I !d-~'.•Ui I~~~.;!:~~~<,7gJ(p 11691 S.19te6 wrvw.sgs r.°m <br />- - ~a.mM d IN sss ao~~ cT«,h~ aw~w a. tonr«~M <br />