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SGT <br />AOOnESS ALL COjiRESPONDENCE TO <br />COAWERCIAL TESTwG 6 EkGINGEn:NG GO. <br />1665 PARIS STREET 6UnE 040p <br />DENVE R. CO tl0239 <br />iLl (.'103) 373772 <br />rA% (,i03) 37]-1791 <br />Table 1 -Colorado Acute Test Conditions <br />Parameter C. dubia 81 D. magna Fathead Minnow <br />Test Type Static Renewal Static Renewal <br />Temperature 20 +/- 1° C 20 +/- 1 ° C <br />Photoperiod 16 hr lighti8 hr dark 16 hr IighUB hr dark <br />Test Chamber Size 30 ml 600 ml <br />Test Sample Volume 20 ml 300 ml <br />Renewal Period 24 hrs 24 hrs <br />Age of Organisms < 24 hrs 1 l0 14 days <br />Number per Chamber 5 10 <br />Chambers Per Concentration 4 4 <br />Feeding Regime Per Chamber 0.1 ml Algae/Y'TC 0.1 ml Brine Shrimp <br />Test Duration 48 hrs 96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic MethodiEquipment EPA Method <br />Alkalinity Titrimetric (APHA6, 1989) EPA 310.1 <br />Ammonia, as Nitrogen Colorimetric Analysis Method EPA 350.1 <br />Chlorine, Total Residual LaMotte STC-CI Colorimeter EPA 330.4 <br />Conductivity Oakton CON 500 Cond. Meter EPA 120.1 <br />Dissolved Oxygen Orion Model 850 DO Meter EPA 360.1 <br />Hardness Titrimetric (APHA, 1989) EPA 130.2 <br />pH Fisher Model 925 pH Meter EPA 150.1 <br />Temperature NIST7 calibrated thermometer EPA 170.1 <br />References <br />American Public Health Association. 1992. Standard Methods for the Examination of Wafer acrd <br />Wastewater, 18'" Edition. American Public Health Association, Washington, D.C. <br />Colorado Department of Health. 1998. Guidelines for Conducting Whole Effluent Toxicity Tests Water <br />Quality Control Division, Denver, Colorado 8024G-1530. <br />USEPA 1993. Methods for Measuring the Acute Toxlcrty of FNluents to Freshwater and Marine <br />Organisms. EPAl600i4-90/027F, 4'" edition. EMSL, C:ncinnali, Ohio A5?.EiB <br />° American Public Health AssoGation <br />' National Institute 01 Standards and Tochnofogy <br />..~ <br />~~ ~:p~aa+ ,. '.I ;~.....~~utra Ii!:~f.7~9700 It6~111453-9706 www sa: wm <br />~~ ~.,..~+°.. scs ccw lsacduawwwiw....°c.i <br />