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i <br />ADOn[ 4:5 AL L CUIIRE S[`ONOEN:.L TO <br />COrANERCinI TESTWG 8 ENGWEERI7 ~ CO. <br />4Fki$ 7AA+5 STREET, S111TF a-200 <br />OENYER. CO 80239 <br />TEL ;3ar37aan2 <br />FA%. ;3N/3TJ~791 <br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Fonn Pg. 6-7 <br />Narrative 8 Discussion Page 4 Reference Toxicity Dala Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' Pg. 10 <br />SGS Commercial Testing and Engineering, Co. conducted a two species acute bioassay on Western <br />Fuels New Horizon Mine effluent discharge. This discharge has been designated as outfall 007. The <br />purpose of this bioassay was to determine if the facility complies with the acute WET portion of the <br />Colorado Stale Industrial General Minimal Discharge Permit. The test organisms utilized were the <br />fathead minnow (Pimephales promelas) and cladoceran (Daphnia magna). Test results indicate that the <br />effluent was not acutely toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is <br />in compliance with the acute WET portion of its Colorado Slate Discharge Permit for the second quarter <br />of 2003. <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine. <br />outfall 007. An effluent grab sample was collected on May 12, 2003, stored on ice, and delivered to SGS <br />Commercial Testing and Engineering, Co. on the following day. The effluent sample was received al a <br />temperature of 2.0 ° C and assigned sample tracking number 072-4930-001. The bioassay was initiated <br />approximately 25 hours after sample collection. <br />Testing was conducted In accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Daphnia magna, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water at 140.185 mg/L CaC03 Control replicates were exposed to 100% laboratory water. <br />Statistically, the Binomial method deterrnined the LC~'s2. Significant differences in mortality were <br />analyzed using the Steel's Many-One Rank test. <br />' Supplemental forms include slatisucal analysis and chain-ol-custody feints <br />° LCao - an eshmate of the concentration lethal to 50°~ 01 the organisms in Iho limo prescribed by the lost <br />G ~ ~ ~, ~ .i ~.~~ t'. ~N!i~rits S°mc°: Ga0aa18 UI[ce <br />19+95 Ngnland Av°. 1~nt°?IOB. lomav° !1 G3tt8 1167019539700 116?e1951•x!W wrnas,tcor•, <br />pan ~ M~+M M fh L°5 Gimp rSaM~ anN.a n SwwwMN <br />