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W.E. T. Inc. <br />Water & Environmental Testinplnc. 235 West 400 South, American Fork, Ulah 84003 (80])763-0660 Fax(80I)763-0440 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: January 5, 2002 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 008 <br />TEST (Animal/Age): Ceriodaphnia <24 hours. SAMPLE (Dale/Type): 12/26/01 Grab. <br />DATE/TIME TEST BEGAN: 12/27/01 2:35 pm <br />DATE/TIME TEST COMPLETED: 12/29/01 2:30 pm <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed [o diluted Effluent as specified by the permit. At the end of [he test period <br />Survivat was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br /><24 hours. <br />5 organisms/15 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light S hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />None was supplied. <br />SUMMARY <br />Results: Pass X Fail <br />There WAS significant effect on survival. LC50 estimated at 55.5%. <br />Enclosed are data sheets and statistical reports. <br />Sincerel , <br />~~~~ <br />ee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />Page 4 of 10 <br />