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W.~'. T. Inc. <br />Water & Environmentat Testine Inc. Z35 West 400 South, American Fork, Utah 84003 (8011 763-0 6 60 Fax(801)763-0440 <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: January 5, 2002 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (Anima!/Age): Fathead Minnow, 6 days. SAMPLE (Date/Type); 12/26/01 Grab. <br />DATE/TIME TEST BEGAN: 12/27/01 2: l5 pm DATE/TIME TEST COMPLETED: 12/31/01 3:45 pm <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Beplicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Fathead Minnow, 6 days. <br />10 organisms/150 mU2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />None was supplied. <br />100%. <br />SUMMARY <br />Results: X~ Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />ee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />Page 2 of 9 <br />