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Table 1 -Colorado Acute Test Conditions <br />Parameter D. Magna Fathead Minnow <br />Test Type Static Renewal Static Renewal <br />Temperature 20 +/- 1° C 20 +/- 1° C <br />Photoperiod 16 hr IighU8 hr dark 16 hr IighU8 hr dark <br />Test Chamber Size 30 ml 600 ml <br />Test Sample Volume 20 ml 300 ml <br />Renewal Period 24 hrs 24 hrs <br />Age of Organisms < 24 hrs 7 days <br />Number per Chamber 5 10 <br />Chambers Per Concentration 4 4 <br />Feeding Regime Per Chamber None o.15 ml Brine Shrimp @ 48 hrs <br />Test Duration 48 hrs 96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic <br />MethodlEquipment <br />EPA Method <br />Alkalinity <br />Ammonia <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />Analytical Balance <br />References <br />Titrimetric <br />CHEMetrics Colorimetery K-1510 <br />CHEMetrics Colorimetery K-2504 <br />Cole-Parmer Model 1500-10 <br />YSI Model 59 DO Meter <br />Titrimetric <br />Fisher Accumet Model 910 pH Meter <br />NISI calibrated thermometer <br />Mettler Model AE 240 <br />EPA 310.1 <br />EPA 120.1 <br />EPA 360.1 <br />EPA 310.1 & 130.2 <br />EPA 150.1 <br />Methods for Chemical Analysis of Water and Waste, Environmental Protection Agency, Environmental <br />Monitoring Systems Laboratory- Cincinnati (EMSL-CI), EPA-600/4-79-020, Revised March 1983. <br />Colorado Department of Health 1998 Guidelines for Conducting Whole Effluent Toxicity Tests Water Quality <br />Control Division, Denver, Colorado 80246-1530. <br />USEPA. October 2002. Methods for Measuring fhe Acute Toxicity of Effluents and Receiving Wafers to <br />Freshwater and Marine Organisms. Fifth Edition. U.S. Environmental Protection Agency, Office of Water, <br />Washington, D.C. EPA-821-R-02-012. <br />e National Institute of Standards and Technology <br />