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Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplemental Forms' Pg. 10 <br />Abstract <br />SGS Minerals Services Division conducted a two species acute bioassay on Western Fuels effluent <br />discharge. This discharge has been designated as outfall 007. The purpose of this bioassay was to <br />determine if the facility complies with the acute WET portion of the Colorado State Permit System <br />Authorization to Discharge. The test organisms utilized were the fathead minnow (Pimephales promelas) <br />and cladoceran (Daphnia Magna). Test results indicate that the effluent was notacutely toxic to the <br />fathead minnow or the Daphnia Magna survival. Therefore, outfa11007 is in compliance with the <br />acute WET portion of its Colorado State Discharge Permit for the firstquarter of 2006. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels, outfall 007. An <br />effluent grab sample was collected on February 20, 2007, stored on ice, and delivered to SGS <br />Environmental Services Division on the next day. The effluent sample was received at a temperature of <br />4.8° C and assigned sample tracking number TA7-BO-P044-001. The bioassay was initiated <br />approximately 24 hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Daphnia Magna, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water having a hardness of 70 mg/L CaCO,. Control replicates were exposed to 100% <br />laboratory water. <br />Statistically, the Binomial method was used to determine the LC~Z for both P. promelas and D. magna. <br />Significant differences in mortality were analyzed using Dunnett's test. <br />Supplemental forms include statistical analysis and chain-of-custody forms <br />s LCso - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />2 <br />