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PART 1 <br />Page No. 5 <br />Permit ~o. CO-OOOOl32 <br />• <br />A. DEFIlYITION OF EFFLUENT LL~IITATIONS <br />2. Acute WET Testing - Outfall 011 <br />(a) Testine and Reporting Requirements <br />Tests shall be done at [he frequency listed m Part [.B.l. Test results shall be reported along with the Discharge <br />\~loni[onng Report (DMR) submitted for the repornue period during which the sample was taken. (i.e., WE7 jesting <br />results for the first calendar quarter endure March 31 shall be reported with the DMR due April 28.) The results shall <br />be submitted on the Acute Toxictry Test report form, available from the Division. Copies of these reports are to be <br />submitted to both the Division and EPA along with the DMR. <br />The permittee shall conduct an acute 96-hour WET test using fathead minnows. Acute tests will be replacement static <br />tests of a single e(Fluent grab sample. The permittee shall conduct each acute WET test in general accordance with <br />methods described in Methods For Ivleasurine the Acute Toxicity of EfFluents and Receiving Waters to Freshwater and <br />Marine Oreantsms, EPA/600/4-90/027 or the most current edition, except as modified by the most current Division <br />gmdance document entitled Guidelines for Conducting Whole EfFluent Toxicity Tests. <br />(b) Failure of Test and Division Notification <br />• <br />An acute WET test is faded whenever the LCso, which represents an estimate of the efluent concentration which is <br />lethal [0 50% of the test organisms in the time period prescribed by [he test, is found to be less than or equal to 100% <br />effluent. The permittee must provide written notification of the failure of a WET test to the Division, along with a <br />statement as to whether the Preliminary Toxicity Incident ('PTi")/Toxicity Identification Evaluation ("TiE") <br />investigation or accelerated testing is being performed (see next section). Notification must be received by the <br />Division within 14 calendar days of the demonstration of acute WET in the routine required test. <br />"Demonstration" for the purposes of Parts I. A.2.(b),(c),(d) and (f) means no later than the last day of the laboratory <br />test. <br />(c) Automatic Compliance Schedule Uoon Failure of Test <br />ff a routine acute WET test is failed, [he following automatic compliance schedule shall apply. As part of [his the <br />permittee shall either: <br />(i) proceed to conduct [he PTI/TIE investigation as described in Par[ [.A.2.(d), or <br />(ii) conduct accelerated testing using the single species found [o be more sensitive. <br />If accelerated testing is being performed, the permittee shall provide written notification of the results within 14 <br />calendar days of completion of the "Pattern of Toxicity"/"No Toxicity" demonstration. Testutg will be at least <br />once every two weeks for up to five tests until 1) two consecutive tests fail or three of five tests fail, in which case a <br />pattern of toxicity has been demonstrated or, 2) two consecutive tests pass or three of five tests pass, m which case no <br />pattern of toxicity has been found. If no pattern of toxicity is found the toxicity episode is considered [o be ended and <br />routine testing is to resume. If a pattern of toxicity is found, a PTIIT[E investigation is to be performed. If a pattern <br />of toxicity is not demonstrated but a significant level of erratic toxicity is Found, the Division may require an increased <br />frequency of routine monitoring or some other modified approach. <br />See Part [.C. for Definitions. <br />~,.~ , <br />-~ cUIIU 9., :,, ., ,: <br />Amendment No. 5 Issued Effective ~ L'J4i~J <br />• <br />