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W.E. T. Inc. ~~~ ~~~~~~~~~~~~~ ~~~ <br />Water & I;nvironmentai Testing lnc. 399 West 1200 North. B Orem. Utah 84057 (801)235-9741 Fax(801)235-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: June 25, 2001 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (Anima!/Age): Ceriodaphnia <24 hours. <br />DATE/I'IME TEST BEGAN: 6/20/01 11:45 a.m. <br />SAMPLE (Dore/Iype): 6/19/01 Grab. <br />DATE/TIME TEST COMPLETED: 6/22/01 11:00 a.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted E(Fluent as specified by the permit. At the end of the test period Survival <br />was measured [o determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start Q4 hours. <br />Number of Organisms/Dilution Volume/Replicates 5 organisms/IS mL/4 replicates. <br />Food Fed YTC and Algae before starting test. <br />Aeration Nane required. <br />Dissolved Oxygen Measured daily old/new. <br />Water Replacement Renewed daily. <br />Temperature 20+ 1 degrees C. <br />Photo Period 16 hours light 8 (tours dark. <br />pH Measured initially and at 24 hours. <br />Dilution Water Reconstituted lab water approx. 200 mg/L hardness. <br />Receiving Water None was supplied. <br />Sample Concentrations Control, 6.25, 12.5, 25, 50,_ 100%. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />Rawlings <br />ater & Environmental Testing, Inc. <br />Enclosure <br />