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~~' ~~~'. ~. InG III ~~,~~~~~~~~~~~~~ <br />Wafer do L~nvlronmenta! Ttstlne Ine 399 West 1100 North. B Orem. Utah 84057 (8 01123 5-9 741 Fax{8011135-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: December 20, 2000 <br />PERMITTEE NAME: Western Fuels Co. <br />TEST (AnimaU.tge): Ceriodaphnia Q4 hours. <br />DATE/I'IME TEST BEGAN: 12/14/00 11:30 e.m. <br />SITE ID: New Horizon Mine OUTFACE NO: 007 <br />SAMPLE (DatellypeJ: 12/13/00 Grab. <br />DATE/I'IME TEST COMPLETED: 12/16/00 12:00 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Anlmal Age at Test Start <br />Number of Organisms/Dilution VolumelReplicates <br />Food <br />4cr:u iun <br />Dissolved Oxygeu <br />Water Replacement <br />Temperature <br />Photo Period <br />PD <br />DButlon Water <br />'Recetvfne Water <br /><24 hours. <br />5 organisms/I S mU4 replicates. <br />Fed YTC and Algae before starting test. <br />~~~nc iryuual <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hotus. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed erc data sheets and statistical rcpoRs. <br />Sincere) `~ // <br />/~/~`,' ` <br />giLJ <br />Rawlioga <br />Water Bc Environmental Testing, Inc. <br />Enclosure <br />