Laserfiche WebLink
,~ <br />METHODS <br />A two species acute bioassay was conducted with effluent from the Lone Pine Pipeline, outfall <br />013. An effluent grab sample w•as collected on ]anuary 20, 2000, stored on ice, and delivered to <br />Commercial Testing and Engineering, Co. (CT&E) on January 21, 2000. The eflluent sample <br />was received at a temperature of 7.0 ° C and assigned CT&E sample tracking number 72-421428. <br />The bioassay was initiated approximately 25 hours after sample collection. <br />r,. <br />'' Testing was conducted in accordance with the most recent protocols specified by the Water <br />_ , Quality Control Division of the Colorado Department of Health (1998), outlined in Table 1. <br />Basic characteristics of the eflluent and dilutions were measured as follows: <br />Characteristic Method/Equipment EPA Method <br />Alkalinity <br />Ammonia <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hazdness <br />pH <br />Temperature <br />Titrimetric (APHAI, 1989) <br />Lachat Analysis Method <br />LaMotte STC-CJ Colorimeter <br />Orion Model 160 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Model 925 pH Meter <br />NIST' calibrated thermometer <br />EPA 310.1 & 130.2 <br />EPA 350.1 <br />EPA accepted <br />EPA 120. ] <br />EPA 360.1 <br />EPA 310.1 & 130.2 <br />EPA 150.1 <br />EPA accepted <br />The tests exposed daphnids, Daphnia magna, to five concentrations of eflluent, 100%, 75%, <br />50%, 25%, and 1.9%, prepazed using reconstituted laboratory water at 110 mg/L CaC03. <br />Additionally, the tests exposed fathead minnows, Pimephalespromelas, to five concentrations of <br />effluent, 100%, 75%, 50°/q ZS%, and 1.9%, prepazed using reconstituted laboratory water at 110 <br />mg/L CaC03. Control replicates were exposed to 100% laboratory water. <br />--, The Binomial method determined the LCso's. Significant differences in mortality were analyaed <br />using Steel's Many-One hank tests. <br /> <br />-~ <br />l <br />American Public Health Association <br />„}. ~ National Ltstitute of Standards and Txhnology <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />F~6° <br />~Onginal Watertnarketl For Your Protection ~, <br />