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Mountain Coal Company CO-0038776 SeaCrest Number: 303251 <br />ACUTE TOXICITY TEST SUMMARY <br />Test: 48-hour static renewal using Daphnia magna and 96-hour static renewal using fathead <br />minnows (Pimephales promelas). <br />Client: Mountain Coal Company <br />Test Procedure Followed: Weber (1993) <br />Sample Description: Discharge 017 <br />Time/Date of Sample Collection: 08:15, July 15, 2003 <br />08:00, July 29, 2003 <br />Time/Date of Sample Receipt: 09:40, July 16, 2003 <br />10:00, July 30, 2003 <br />Test Initiation Time/Date: Fathead minnows: 15:30, July 16, 2003 <br />Daphnia magna: 14:30, July 30, 2003 <br />Test Completion Time/Date: Fathead minnow test: 15:00, July 20, 2003 <br />Daphnia magna test: 14:45, August 1, 2003 <br />Dilution Water: Moderately-hard reconstituted freshwater <br />Test Organism Source: Daphnia magna -The SeaCrest Group <br />Fathead minnows -Aquatic BioSystems, Inc. <br />Reference Toxicant: Sodium chloride <br />The SeaCrest Group 5 <br />