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<br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' Pg. 10 <br />Abstract <br />SGS Minerals Services Division conducted a two species acute bioassay on Western Fuels New Horizon <br />Mine effluent discharge. This discharge has been designated as outfall 007. The purpose of this <br />bioassay was to determine if the facility complies with the acute WET portion of the Colorado State <br />Industrial General Minimal Discharge Permit. The test organisms utilized were the fathead minnow <br />(Pimephales promelas) and cladoceran (Daphnia magna). Test results indicate that the effluent was not <br />acutely toxic to fathead minnow and Daphnia magna survival. Therefore, ouffall 007 is in compliance <br />with the acute WET portion of its Colorado State Discharge Permit for the fourth quarter of 2004. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />outfall 007. An effluent grab sample was collected on October 19, 2004, stored on ice, and delivered to <br />SGS Minerals Services Division on the same day. The effluent sample was received at a temperature of <br />1.0 ° C and assigned sample tracking number 072-11340-001. The bioassay was initiated approximately <br />2 hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Daphnia magna, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water at 115-160 mglL CaCO3. Control replicates were exposed to 100% laboratory water. <br />Statistically, the Binomial method determined the LCSO's2. Significant differences in mortality were <br />analyzed using the Steel's Many-One Rank test. <br />' Supplemental forms include statistical analysis and chain-of-custody forms <br />2 LCD - an estimate of the concentration Lethal to 50% of the organisms in the time prescribed by the test <br />F-526 <br />