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D. Magna Western Fuels 007 10-19-04 1240 Program run on 10-26-2004 at 13:48:07 RF~ULTS CALCULATED USING THE BINOMINAL METHOD CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD - - - PROB.(PERCENT) - - - -- - - - --------- 100 --------------- 20 -------------- 1 ---- -- ----- 5.00 ------ ----- -- -- -- -- - 0.0020 75 20 0 0.00 LESS THAN 0.001 50 20 0 0.00 LESS THAN 0.001 25 20 0 0.00 LESS THAN 0.001 12.5 20 0 0.00 LESS THAN 0.001 AT A CONFIDENCE LEVEL OF 95 PERCENT, THE BINOMIAL TEST SHOWS THAT THE LC50 IS ABOVE 100 THE USEFULNESS OF ANY LC50 CALCULATED FROM THIS SET OF DATA IS QUESTIONABLE BECAUSE A CONCENTRATION-EFFECT RELATIONSHIP HAS NOT BEEN DEMONSTRATED OVER A REASONABLE RANGE (e.g. <37 TO>63 OF PERCENT DEAD NEITHER THE APPROXIMATE LC50 CALCULATION NOR THE MOVING AVERAGE METHOD CAN BE USED WITH THIS SET OF DATA. EITHER THE HIGHEST CONCENTRATION KILLED LESS THAN 50 PERCENT OR THE LOWEST KILLED MORE THAN 50 PERCENT. IF THE PROBIT SLOPE IS NEGATIVE, ENTER THE DATA AGAIN USING THE NUMBER ALIVE INSTEAD OF THE NUMBER DEAD. W._~N THERE ARE LESS THAN TWO DIFFERENT CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, THE PROBIT METHOD CANNOT GIVE ANY STATISTICALLY SOUND RESULTS. COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE REASONABLE.