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Mountain Coal Company CO - 0038766 SCG Project No.: 304222 <br />Site: West Elk Mine <br />The SeaCrest Group <br />Quality Assurance Check List -Acute and Chronic Whole Effluent Toxicity Test <br />Client: Mountain Coal Company SeaCrest Sample No.: 304222 <br />Species Tested: Daphnia magna and Fathead minnow <br />Start Date of Test , Start Date of Test <br />(Daphnia magna) (Fathead minnow) <br />8-26-04 8-26-04 <br />Sample received in lab properly preserved (4°C)? N* <br />Sample delivered on ice or equivalent? Y <br />Test initiated within 36-hours of collection? Y <br />Test protocol conforms to CDPHE guidelines (Daphnia magna)? Y <br />Test protocol conforms to CDPHE guidelines (Fathead minnow)? Y <br />Average test temp. f 1°C (Daphnia magna)? Y <br />Average test temp. f 1 °C (Fathead minnow)? Y <br />DO level >4.Omg/I; no super-saturation (Daphnia magna)? Y** <br />DO level >4.Omg/1; no super-saturation (Fathead minnow)? Y <br />Mortality in control <10%, <20% for chronic (Daphnia magna)? Y <br />Mortality in control <10%, <20% for chronic (Fathead minnow)? Y <br />Daphnia magna neonates in control <24-hours old? Y <br />Fathead minnow larvae in control 1 to 14 days old? Y <br />Appropriate reference toxicity test conducted? Y <br />Lab. Ref. Tox. test results within the confidence limits for the lab? Y <br />*Temperature of effluent above the EPA recommended 6°C upon delivery to SeaCrest lab. <br />* *DO in 100% effluent high after 24-hour renewal. Aerated effluent in order to reduce DO. <br />Position: Senior Scientist <br />Z <br />The SeaCrest Group 13 <br />