HYDRO27761

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 11/1/2004
• Doc Name: Biomonitoring Report Dated September 22, 2004
• From: SeaCrest Group
• To: Mountain Coal Company
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

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The SeaCrest Group Daphnid Acute Benchsheet Form #: 102a .r ~
Louisville, CO Effective: February 2002
Permittee: MT. C,xv Site: W. Elk M~n¢. lab#: 30y 2'L2
IWC %: (, Q Template #: '~ Sample Date: q~q 2rioy Dilution Water: Mi-{
Age & Source: cf3'O.c,04 -x,14 c oral Test Started pc>,.>~„p~- 13W Test Ended: (7~y ~t}p y - ! jty ~;
Test Conditions: 12.5"io D~~u.~r~or'/
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1. Exposure Chamber
Total Capacity: 30 ml Test Solution Volume: 15 ml
Test Solution Surface Area: cm` Water Depth (constant): cm
(cyclic): cm
2. Feeding Schedule
Not Fed: Fed Daily:
Fed Irregularly: X Food Used: YCT, Algae
(describe): fed 1-2 hours before test
3. Aeration
None:
Before Use: ~_minutes @ -100 bubbles/min)
4. Screened Animal Enclosures
Not Used: X Used: cm diameter
5. Condition/appearance of surviving organisms at end of test (i.e., alive but immobile, loss of
orientation, erratic movement, etc): A~lv'~ r ~izc~e/c~
6. Comments: ~,F°~~,w~t;~, iN ~~ , 5u rc~hri~w e~~S~;:~~ ~~~;'.~c,I/r