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~G~ Table of Contents Abstract Page 2 Table 2 -Methods and Equipment Page 5 Methods Page 2 References Page 5 Results Page 3 Discharge Monitoring Report Form Page 6 Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Page 7 Table 1 -Colo. Test Conditions Page 5 Supplemental Fonns' Page 10 Abstract In March 2006, a two species acute bioassay was conducted with effluent from the Western Fuels, outfall 007. The purpose of the bioassay was to determine if the facility complies with the acute WET portion of the Colorado State Permit System Authorization to Discharge. According to the Western discharge permit (CO-0000213), an acute WET test is failed whenever the LCD, which represents ari estimate of the effluent concentration which is lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less than or equal to 100% effluent. Results of the testing indicated the sample was acutely toxic to fathead minnow survival but not to Daphnia magna survival. To determine if the facility complies with the "Automatic Compliance Schedule" of the acute WET portion of the Colorado State Permit System Authorization to Discharge, SGS North America Inc. conducted an acre/ereted one specie acute bioassay on the Western Fuels effluent discharge. The test organism utilized was the fathead minnow (Pimephales promelas). The results for the first sample of the accelerated testing schedule indicate this effluent sample was not acutely toxic to fathead minnow survival. Methods A one specie accelerated acute bioassay was conducted with effluent from the Western Fuels, outfall 007. An effluent grab sample was collected on April 19, 2006, stored on ice, and delivered to SGS North America Inc. on the next day. The effluent sample was received at a temperature of 1 ° C and assigned sample tracking number 072-19111-001. The bioassay was initiated approximately 27 hours after sample collection. Testing was conducted in accordance with the most recent protocols specified by the Water Quality Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. Statistically, the Binomial method was used to determine the LCso2 for P. promelas. Significant differences in mortality were analyzed using the Steel's Many-One Rank test. ' Supplemental forms include statistical analysis and chain-ofcustody forms z LCD - an esfimate of the concentration lethal to 50% of the organisms in the time prescribed by the test