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Western Fuels Accelerated Fathead Acute #2 <br />Program run on 05-18-2006 at 14:22:32 <br />RESULTS CALCULATED USING THE BINOMINAL METHOD <br />---------- <br />CONC. --------------- <br />NUMBER ------------- <br />NUMBER --------------- <br />PERCENT --------------------------- <br />BINOMIAL <br /> EXPOSED DEAD DEAD PROB.(PERCENT) <br />100 40 3 7.50 LESS THAN 0.001 <br />75 40 5 12.50 LESS THAN 0.001 <br />50 40 5 12.50 LESS THAN 0.001 <br />25 40 1 2.50 LESS THAN 0.001 <br />12.5 40 1 2.50 LESS THAN 0.001 <br />AT A CONFIDENCE LEVEL OF 95 PERCENT, THE BINOMIAL TEST <br />SHOWS THAT THE LC50 IS ABOVE 100 <br />THE USEFULNESS OF ANY LC50 CALCULATED FROM THIS SET OF DATA <br />IS QUESTIONABLE BECAUSE A CONCENTRATION-EFFECT RELATIONSHIP <br />HAS NOT BEEN DEMONSTRATED OVER A REASONABLE RANGE (e.g. <br /><37 TO>63 OF PERCENT DEAD <br />NEITHER THE APPROXIMATE LC50 CALCULATION NOR THE MOVING <br />AVERAGE METHOD CAN BE USED WITH THIS SET OF DATA. <br />EITHER THE HIGHEST CONCENTRATION KILLED LESS THAN 50 <br />PERCENT OR THE LOWEST KILLED. MORE THAN 50 PERCENT. <br />IF THE PROBIT SLOPE IS NEGATIVE, ENTER THE DATA AGAIN <br />USING THE NUMBER ALIVE INSTEAD OF THE NUMBER DEAD. <br />RESULTS CALCULATED USING THE PROBIT METHOD <br />----------- ------------------- ------------------ -------------------------------- <br />GOODNESS OF FIT <br />ITERATIONS <br />----------- G <br />------------------- H <br />-- <br />-- <br />- <br />- <br />- CHI-SQUARE PROBABILITY <br />------------------------- <br />- <br />-- <br />4 1.170 -- <br />- <br />-- <br />--- <br />-- <br />- <br />1.000 - <br />-- <br />- <br />2.738 0.434 <br />SLOPE _ .8318566 <br />95 PERCENT CONFIDENCE LIMITS = -6.797212E-02 A ND 1.731685 <br />LC50 = 2547.972 <br />95 PERCENT CONFIDENCE LIMITS = 315.7434 AND + INFINITY <br />LC1 = 4.070795 <br />95 PERCENT CONFIDENCE LIMITS = 0 AND 16.80236 <br />COMPARE RES ULTS WITH ORIGINAL DATA TO SEE IF THEY ARE <br />REASONABLE. <br />