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Mountain Coal CO-0038776 <br />SeaCrest Sample No.: 304146 <br />The SeaCrest Group <br />Quality Assurance Check List -Acute and Chronic Whole Effluent Toxicity Test <br />Client:Mountain Coal <br />Species Tested: <br />Daphnia magna <br />Start Date of Test Start Date of Test <br />(Daphnia magna) (Fathead minnow - Pimephales promelas) <br />June 16,2004 N/A <br />Sample received in lab properly preserved (4°C)? N* <br />Sample delivered on ice or equivalent? Y* <br />Test initiated within 36-hours of collection? Y <br />Test protocol conforms to CDPHE guidelines (Daphnia magna)? Y <br />Test protocol conforms to CDPHE guidelines (Fathead minnow - P. promelas)? N/A <br />Average test temp. tl°C (Daphnia magna)? Y <br />Average test temp. fl°C (Fathead minnow -Pimephales promelas)? N/A <br />DO level >4.Omg/1; no super-saturation (Daphnia magna)? Y <br />DO level >4.Omg/1; no super-saturation (Fathead minnow-P. promelas)? N/A <br />Mortality in control <10%, <20% for chronic (Daphnia magna)? Y <br />Mortality in control <10%, <20% for chronic (Fathead minnow - P. promelas)? N/A <br />Ceriodaphnia dubia neonates in control <24-hours old? Y <br />Fathead minnow (P. promelas) larvae in control 1 to 14 days old? N/A <br />Appropriate reference toxicity test conducted? Y <br />Lab. Ref. Tox. test results within the confidence limits for the lab? Y <br />*Sample at temperature above the required 4 degree C upon arrival to SeaCrest <br />laboratory. Sample was packed on blue ice. <br />Position: Senior Scientist <br />SCG Project Number: 304146 <br />6/6 ~ <br />The SeaCrest Group, LLC 14 <br />