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W.~'. T. Inc. <br />Water & Environmental Testine Inc 399 West 1200 North. B Orem, Ufah 84057 (8 01113 5-9 74 1 Fax(8011135-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnta <br />DATE: September 27, 2000 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (AnlmaUAgeJ: Ceriodaphnta <24 hours. SAMPLE (Dale/7ype): 7/20/00 Grab. <br />DATElI'IME TEST BEGAN: 7/21/00 12:15 p.m. DATE/TIME TEST COMPLETED: 9f23/00 1:30 p.m. <br />TEST CONDITIONS <br />Ceriodaphnta dubia neonates were exposed to diluted EfFluent as specified by the permit. At the end of the test period Survival <br />was meazured to determine if Acute Toxicity was present in the sample. <br />Anima{ Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />PFI <br />Dilution Water <br />Receiving Water <br />Sam le Concenti <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, i ~ <br />Rawlings <br />Water & Environmental Testing, Inc. <br /><l4 hours. <br />5 organisms/I S mU4 replicates. <br />Fed YTC and Algae before s~tartitig test. <br />None required. <br />Measured daily old/new: i ~ ... <br />Renewed daily. <br />20 + I degrees C. ; <br />16 hours light 8 hours dark. <br />Measured initially end at 24 hours.. ':: <br />Reconstituted water (+/~ I S% hazdness of the receiving <br />water). <br />None was supplied. ~ <br />Enclosure <br />