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i'i'.~'. T. Inc. <br />Water do Environmenta! TestinY /ne. 399 West 1100 North. 8 Orem. Utah 84057 (801123 5-9 741 Fax(80/1135-9560 <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: September 27, 2000 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (AnimaUAge): Fathead Minnow, 7 days. SAMPLE (Date/Type): 9!20/00 Grab. <br />DATE/I'IME TEST BEGAN: 9/21/00 12:45 p.m. DATE/I'IME TEST COMPLETED: 925/00 12:15 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted efFluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animsl Age at Test Start Fathead Minnow, 7. days: ;. <br />Number ofOrganisms/Dilution VolumelReplicates 10 organisms/I50 ml.l2 replicates <br />.Food Fed.before starting test and tit48 hours; newly:hatched <br /> brine shriinp. F <br />Aeration None required. <br />Dissplved Oxygen Measured daily old/new. ~ <br />Water Replacement Renewed daily. <br />Temperature ' 20 ± I degrees.C. ' <br />Photo Period 16 hours light 8 hours dark. <br /> <br />pH .. <br />Measured initially and at 24 hours. <br />Dilution Water Reconstituted water (+/- 15% hardness of the receiving <br /> water). <br />Receiving.Water None was supplied. <br />Sample Concentrations ___ Control, 6.25, 12.5, 25, 50, 100%. <br />SUMMARY <br />Results: X. Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely '~ ~ - <br />Rawlingsv,',',r,/'" <br />Water & Environmental Testing, Inc. <br />Enclosure <br />