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III IIIIIIIIIIIIIIII <br />~~~YE. T. rn~. 9~ <br />Rarer dr Bnvfronmenta! TesNnr Inu 399 WtsY 1200 Norl6. B Or~m. Utak 8(057 /801)235-97(1 Fax(8011235-9560 <br />~~ <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: December 20, 2000 <br />PERMITTEE NAME: Western Fuels Co. <br />TEST (AnrmaUAgeJ: Fathead Minnow, 6 days. <br />DATE/I'IME TEST BEGAN: 12JI3/00 12:00 p.m <br />SITE ID: New Horizon Mine OUTFACE NO: 007 <br />SAMPLE (Date/type): 12/13/00 Grab. <br />DATE/I'IME TEST COMPLETED: l2/I8/00 12:00 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent es specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Anlmd Age at Test Start <br />Number of Organlsms/Dilutlon Volume/Replicates <br />Fn~ri <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />PH <br />DIIuHon Water <br />Reeelving Water <br />Fathead Minnow, 6 days: <br />10 organisms/ 150 mU2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />hriue .hrin <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initiallyand. at24,hours .:. : . .:.:::. :..: <br />Reconstituted lab water approx. 200 mg/L hardness. <br />None was supplied. <br />SUMMARY <br />Resalb: X Pass Fail <br />Thera was NO significant effect on survival. <br />Haclosed ere date sheets and statistical reports. <br />Sincere) ~ ` ,, <br />Rawlings <br />Water & Pavironntental Testing, Inc. <br />Baclotrrue <br />