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Enviro-Chem Analytical, Inc. Acute Toxicity Testing <br />685 West Gunnison Avenue, Suite 108 Trapper Mining -February 2004 <br />Grand Junction, CO 81505 Outfall 002 1 Species <br />Study Summary <br />An effluent sample collected from the Trapper Mine Craig, Colorado was <br />evaluated for the presence of acute toxicity to larval fathead minnows <br />(Pimephales promelas) during Feb. 10-14, 2004. Moderately hard reconstituted <br />water (MHW) prepared according to USEPA guidelines was used as the diluent <br />for the toxicity tests. <br />The effluent tests met minimum control organism performance requirements <br />defined by the USEPA. <br />The table below summarizes the results of the effluent tests. <br />'i ', Fathead Minnow I <br />I Test Endpoint ', (°,6 Efltuenq i <br />I <br />I <br />~ LC50 i >100 <br />Organisms were purchased from Aquatic Biosystems located in Fort Collins, <br />Colorado. AB reference toxicity test for both species were perforated by them <br />and met minimum performance requirements defined by the USEPA and <br />produces IC25 values within the expected ranges. Organisms were or <br />acceptable quality and proper age upon arrival and beginning of test. <br />