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Mountain Coal Company CO-0038776 SeaCrest Number: 303130 <br />ACUTE TOXICITY TEST SUMMARY <br />Test: 48-hour static renewal using Daphnia magna and 96-hour static renewal using fathead <br />minnows (Pimephales promelas). <br />Client: Mountain Coal Company <br />Test Procedure Followed: Weber (1993) <br />Sample Description: Discharge 017 <br />Time/Date of Sample Collection: 10:30, April 1, 2003 <br />Time/Date of Sample Receipt: 09:40, April 2, 2003 <br />Test Initiation Time/Dafe: Fathead minnows: 14:00, Apri12, 2003 <br />Daphnia magna: 16:30, April 2, 2003 <br />Test Completion Time/Date: Fathead minnow test: 13:00; April 6, 2003 <br />Daphnia magna test: 16:30, April 4, 2003 <br />Dilution Water: Moderately-hard reconstituted freshwater <br />Test Organism Source: Daphnia magna -The SeaCrest Group <br />Fathead minnows -The SeaCrest Group <br />Reference Toxicant: Sodium chloride <br />The SeaCrest Group 5 <br />