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Mountain Coal Company CO-0038776 SCG Project No.: 304181 <br />Acute Toxicity Test Summary <br />Test: 48-hour static renewal using Daphnia magna <br />Client: Mountain Coal Company <br />Test Procedure Followed: EPA-821-R-02-012 (2002) <br />Sample Description: 304181 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection Receipt Receipt <br />0800 7/21/04 1230 7/22/04 <br />Test Initiation • Daphnia magna: <br />Time/Date: (Untreated effluent) 154 5 - 7/22/04 <br /> (12.5% dilution) 1600 - 7/22/04 <br /> (25% dilution) 1630 - 7/22/04 <br /> (50% dilution) 1615 - 7/22/04 <br />Test Completion • Daphnia magna: <br />Time/Date: (Untreated effluent) 160 0 - 7/24/04 <br /> (12.5% dilution) 1630 - 7/24/04 <br /> (25% dilution) 1615 - 7/24/04 <br /> (50% dilutionl 1615 - 7/24/04 <br />water <br />Test Organism Source: . Daphnia magna <br />The SeaCrest Group, LLC <br />Reference Toxicant: Sodium Chloride <br />The SeaCrest Group, LLC 3 <br />