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THE SEACREST GROUP <br />puality Assurance Check List -Acute and Chronic Whole Effluent Toxicity Test <br />Client: Mountain Coal <br />Start Date of Test: December 11, 1998 <br />SeaCrest Sample Number: 398410 <br />Species Tested: Daphnia magna <br />Sample received in lab properly preserved (4°C)? N' <br />Sample delivered on ice or equivalent? Y <br />Test initiated within 36 hours of collection? Y <br />Test protocol conforms with CDH guidelines: D. magna? Y <br />Test protocol conforms with CDH guidelines: P. promelas? NIA <br />Average Test Temp ± 1 °C: D. magna? Y <br />Average Test Temp ± 1°C: P. promelas? N/A <br />DO level >4.5 mg/L; no supersaturation: D. magna? Y <br />DO level >4.5 mg/L; no supersaturation: P. promelas? N/A <br />Mortality in control <10%, <20% for chronic: D. magna? Y <br />Mortality in control <10%, <20% for chronic: P. promelas? N/A <br />D. magna neonates in control <24 hours old? Y <br />P. promelas larvae in control 1-14 days old in acute, < 1 day old in chronic? NIA <br />Appropriate reference toxicity test conducted? Y <br />Lab Ref. Tox. test results within the confidence limits for the lab? Y <br />' Sample not at 4°C. <br />Date ~~ l 34 /S~ <br />